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Journal
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Medicine
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Determining Respiration in Human Sperm Cells by High-Resolution Respirometry
/
Respirométrie à haute résolution - Analyse OXPHOS pour les spermatozoïdes humains
JoVE Journal
Medicine
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JoVE Journal
Medicine
High-Resolution Respirometry – OXPHOS Analysis for Human Spermatozoa
Please note that all translations are automatically generated.
Click here for the English version.
Respirométrie à haute résolution - Analyse OXPHOS pour les spermatozoïdes humains
Determining Respiration in Human Sperm Cells by High-Resolution Respirometry
DOI:
10.3791/200320-v
•
05:03 min
•
June 23, 2023
•
Pilar Irigoyen
,
Rossana Sapiro
3
,
Adriana Cassina
3
1
Departamento de Histología y Embriología
,
Unidad Académica Histología y Embriología
,
2
Departamento de Bioquímica
,
Unidad Académica Bioquímica
,
3
Centro de Investigaciones Biomédicas (CEINBIO), Facultad de Medicina
,
Universidad de la República
Tags
Keywords: High-resolution Respirometry
OXPHOS Analysis
Human Spermatozoa
Respirometer
VatLab
Oxygen Sensor Calibration
Cell Preparation Medium
Oxygen Concentration
Respiratory Assessment
Permeabilized Cell Analysis
Digitonin
Substrates
Complex I
Complex II
ADP
Oligomycin
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