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Journal
/
Biology
/
Isolation and Profiling of Single Nuclei from Frozen Mammalian Tissues
/
从冷冻哺乳动物组织中分离出的单核的清理和计数
JoVE Journal
Biology
This content is Free Access.
JoVE Journal
Biology
Clean-Up and Counting of Isolated Single Nuclei from Frozen Mammalian Tissues
Please note that all translations are automatically generated.
Click here for the English version.
从冷冻哺乳动物组织中分离出的单核的清理和计数
Isolation and Profiling of Single Nuclei from Frozen Mammalian Tissues
DOI:
10.3791/200322-v
•
03:38 min
•
July 28, 2023
•
Lucas Stalder
,
Fabian Koechl
,
Kerstin Hahn
,
Marc Sultan
,
Megana K. Prasad
1
Roche Pharma Research and Early Development, Pharmaceutical Sciences
,
Roche Innovation Centre Basel
Tags
Keywords: Single Nuclei
Sucrose Gradient Cleanup
Nuclei Suspension
SCS
Centrifugation
Propidium Iodide Staining
Cell Counter
Single Nuclei RNA Sequencing
Gene Counts
UMI Counts
Mitochondrial Content
Cellular Proportions
Technical Variability
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