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Opprydding og telling av isolerte enkeltkjerner fra frosne pattedyrvev
JoVE Journal
Biology
This content is Free Access.
JoVE Journal
Biology
Clean-Up and Counting of Isolated Single Nuclei from Frozen Mammalian Tissues
Opprydding og telling av isolerte enkeltkjerner fra frosne pattedyrvev
DOI:
10.3791/200322-v
•
03:38 min
•
July 28, 2023
•
Lucas Stalder
,
Fabian Koechl
,
Kerstin Hahn
,
Marc Sultan
,
Megana K. Prasad
1
Roche Pharma Research and Early Development, Pharmaceutical Sciences
,
Roche Innovation Centre Basel
Tags
Single Nuclei
Sucrose Gradient Cleanup
Nuclei Suspension
SCS
Centrifugation
Propidium Iodide Staining
Cell Counter
Single Nuclei RNA Sequencing
Gene Counts
UMI Counts
Mitochondrial Content
Cellular Proportions
Technical Variability
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