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Journal
/
Biochemistry
/
Isolating Small Extracellular Vesicles and Extracting Their Peptidome
/
분리된 작은 세포외 소포(sEV)에서 펩타이드 추출
JoVE Journal
Biochemistry
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JoVE Journal
Biochemistry
Extraction of Peptides from Isolated Small Extracellular Vesicles (sEVs)
Please note that all translations are automatically generated.
Click here for the English version.
분리된 작은 세포외 소포(sEV)에서 펩타이드 추출
Isolating Small Extracellular Vesicles and Extracting Their Peptidome
DOI:
10.3791/200348-v
•
03:04 min
•
June 30, 2023
•
Jiale Cheng
2
,
Jiong Zhu
2
,
Yujiao Liu
3
,
Chen Yang
3
,
Yuehui Zhang
,
Yuchen Liu
,
Chaozhi Jin
,
Jian Wang
2
1
School of Basic Medical Sciences
,
Anhui Medical University
,
2
State Key Laboratory of Proteomics, Beijing Proteome Research Center, National Center for Protein Sciences (Beijing)
,
Beijing Institute of Lifeomics
,
3
School of Life Sciences
,
Hebei University
Tags
Peptide Extraction
Small Extracellular Vesicles
SEVs
Ultracentrifugation
Protease Inhibitor
Ultrasonic Crushing
Dithiothreitol
Iodoacetamide
Ultrafiltration
Desalting
Trifluoroacetic Acid
Acetonitrile
Mass Spectrometry
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