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Intracellular Signaling Events and Hormone Secretion in Human Pseudoislets

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Synchronous Assessment of Fluorescent Biosensor Dynamics and Hormone Profiles in Human Pseudoislets

JoVE Journal
Biology

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JoVE Journal Biology

Synchronous Assessment of Fluorescent Biosensor Dynamics and Hormone Profiles in Human Pseudoislets

Synchronous Assessment of Fluorescent Biosensor Dynamics and Hormone Profiles in Human Pseudoislets

Intracellular Signaling Events and Hormone Secretion in Human Pseudoislets

DOI:

10.3791/200361-v

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06:38 min

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November 03, 2023

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Tiffany M. Richardson*¹, Yasminye D. Pettway*¹, John T. Walker, Heather A. Nelson, Matthew Ishahak, Gregory Poffenberger, Radhika Aramandla, Conrad Reihsmann, Ashutosh Agarwal, Alvin C. Powers^2,4, Marcela Brissova

¹Department of Molecular Physiology and Biophysics,Vanderbilt University School of Medicine, ²Division of Diabetes, Endocrinology and Metabolism, Department of Medicine,Vanderbilt University Medical Center, ³Department of Biomedical Engineering,University of Miami, ⁴VA Tennessee Valley Healthcare System

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Keywords: Fluorescent Biosensor Hormone Profiles Human Pseudoislets Microperfusion Microchip Secretagogue Brightfield Darkfield Pseudo Eyelets Fraction Collector Environmental Chamber Confocal Microscope Efflux Pump Medium Flow

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