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Journal
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Biology
/
Creating Robust Bisulfite-mRNA Libraries for Transcriptome-Wide Mapping
/
用于二代测序的Poly（A） RNA纯化和亚硫酸氢盐转化
JoVE Journal
Biology
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JoVE Journal
Biology
Poly(A) RNA Purification and Bisulfite Conversion for Next Generation Sequencing
Please note that all translations are automatically generated.
Click here for the English version.
用于二代测序的Poly（A） RNA纯化和亚硫酸氢盐转化
Creating Robust Bisulfite-mRNA Libraries for Transcriptome-Wide Mapping
DOI:
10.3791/200389-v
•
05:45 min
•
July 07, 2023
•
Szu-Ying Chen
2
,
Po-Hsien Huang
2
1
Institute of Basic Medical Sciences, College of Medicine
,
National Cheng Kung University
,
2
Department of Biochemistry and Molecular Biology, College of Medicine
,
National Cheng Kung University
Tags
Poly(A) RNA Purification
Bisulfite Conversion
Next-generation Sequencing
Total RNA
Oligo(dT) Beads
Lysis Buffer
Wash Buffer
Tris-HCl
RNA Elution
Magnetic Separation
RNA Sample Preparation
RNA Enrichment
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用于二代测序的Poly（A） RNA纯化和亚硫酸氢盐转化
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