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Hormonal, Metabolic, and Electrical Regulation of Isolated Hypothalamic Neurons

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Separazione magnetica e selezione negativa di colture neuronali murine

JoVE Journal
Neuroscience

This content is Free Access.

JoVE Journal Neuroscience

Magnetic Separation and Negative Selection of Murine Neuronal Cultures

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Separazione magnetica e selezione negativa di colture neuronali murine

Hormonal, Metabolic, and Electrical Regulation of Isolated Hypothalamic Neurons

DOI:

10.3791/200391-v

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02:43 min

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August 04, 2023

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Kathera-Ibarra-Forzisi Elena, Christian Roser, Anika Gaur, Federico Sesti

¹Department of Neuroscience and Cell Biology, Robert Wood Johnson Medical School,Rutgers University

Tags

Magnetic Separation Negative Selection Murine Neuronal Cultures Brain Cell Suspension Non-neuronal Cell Biotin Antibody Anti-biotin Microbeads HBSS/BSA Solution Neurite Formation Axonal Extensions Dendritic Processes Immunofluorescent Studies Microtubule Associated Protein 2 POMC Expression Synaptic Connectivity Hypothalamic Neuronal Populations

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