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Journal
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Bioengineering
/
Closed System Production of Adherent Cells Based on Dissolvable Microcarriers
/
人间充质干细胞从 5 升生物反应器到 15 升生物反应器的两层扩增和 15 升生物反应器的自动化细胞配方
JoVE Journal
Bioengineering
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JoVE Journal
Bioengineering
Two-Tiered Expansion of Human Mesenchymal Stem Cells from 5-Liter to 15-Liter Bioreactors and Automated Cell Formulation from a 15-Liter Bioreactor
Please note that all translations are automatically generated.
Click here for the English version.
人间充质干细胞从 5 升生物反应器到 15 升生物反应器的两层扩增和 15 升生物反应器的自动化细胞配方
Closed System Production of Adherent Cells Based on Dissolvable Microcarriers
DOI:
10.3791/200420-v
•
06:10 min
•
July 07, 2023
•
Yuyang Chen*
1
,
Huanye Xu*
2
,
Yuanyuan Zhang*
2
,
Leilei Guo
,
Miaomiao Lan
,
Yanping Yang
,
Wei Liu
,
Xiaojun Yan
,
Yanan Du
1
Department of Biomedical Engineering, School of Medicine
,
Tsinghua University
,
2
Beijing CytoNiche Biotechnology Co. Ltd.
Tags
Mesenchymal Stem Cells
Bioreactor
Cell Expansion
Automated Cell Formulation
Cell Harvest
Cell Processing
Microcaririer
Serum-free Medium
Cell Inoculation
Medium Supplement
Medium Exchange
Cell Sampling
Cell Harvest
Cell Suspension
Cell Washing
Cell Culture
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