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Cancer Research
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Genome-Scale Screening to Identify and Compare Therapeutic Targets in Tumors
/
게놈 스케일 shRNA 스크리닝을 위한 렌티바이러스 패키징 및 바이러스 적정
JoVE Journal
Cancer Research
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JoVE Journal
Cancer Research
Lentiviral Packaging and Viral Titering for Genome-Scale shRNA Screens
Please note that all translations are automatically generated.
Click here for the English version.
게놈 스케일 shRNA 스크리닝을 위한 렌티바이러스 패키징 및 바이러스 적정
Genome-Scale Screening to Identify and Compare Therapeutic Targets in Tumors
DOI:
10.3791/200477-v
•
03:15 min
•
August 25, 2023
•
Brittany Turner-Ivey
,
Jody Fromm Longo
,
Dorea P. Jenkins
,
Stephen T. Guest
,
Steven L. Carroll
2
1
Hollings Cancer Center
,
Medical University of South Carolina
,
2
Department of Pathology and Laboratory Medicine
,
Medical University of South Carolina
,
3
Department of Computational Medicine and Bioinformatics
,
University of Michigan, Ann Arbor
Tags
Lentiviral Packaging
Viral Titering
Genome-scale ShRNA Screens
293T Cells
Packaging Plasmid Mix
Plasmid Barcode Library
Transfection Reagent
Viral Particles
Viral Titering
Cationic Polymer
Tumor Cell Growth Media
Tumor Cells
Lentivirus
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악성 말초 신경초 종양의 마우스 모델에서 치료 표적을 식별하기 위한 유전자 프로파일링 및 게놈 규모 드롭아웃 스크리닝
게놈 스케일 shRNA 스크리닝을 위한 렌티바이러스 패키징 및 바이러스 적정
게놈 스케일 shRNA 스크리닝을 위한 MPNST 세포의 렌티바이러스 감염 및 게놈 DNA 분리
MPNST 세포의 세포분석기 분석은 치료제에 도전합니다.
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