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Journal
/
Immunology and Infection
/
Neutrophil Functional Assays for Screening Different Signaling Pathways
/
Nitrotetrazolium Blue Chloride (NBT) Assay for Evaluating ROS Production (Video) | JoVE
JoVE Journal
Immunology and Infection
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JoVE Journal
Immunology and Infection
Nitrotetrazolium Blue Chloride (NBT) Assay for Evaluating ROS Production
Nitrotetrazolium Blue Chloride (NBT) Assay for Evaluating ROS Production (Video) | JoVE
Neutrophil Functional Assays for Screening Different Signaling Pathways
DOI:
10.3791/200484-v
•
02:59 min
•
February 09, 2024
•
Isabelle Souza Luz
,
Raquel Takaya
,
Daiane Gonzaga Ribeiro
,
Natanael Sales Silva
,
Lucas Fontes
,
Mariana S. Castro
,
Wagner Fontes
1
Laboratory of Protein Chemistry and Biochemistry, Department of Cell Biology
,
University of Brasilia
,
2
Institute of Physics
,
University of Brasilia
Tags
Keywords: NBT Assay
ROS Production
Polymorphonuclear Neutrophils
DMSO
HBSS
PMA
FMLP
NBT Slide Test
NBT Spectrophotometry Assay
Formazan Deposits
SDS
Sonication
Absorbance Measurement
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