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Journal
/
Immunology and Infection
/
Neutrophil Functional Assays for Screening Different Signaling Pathways
/
Ensaio de cloreto de azul de nitrotetrazólio (NBT) para avaliação da produção de ROS
JoVE Journal
Immunology and Infection
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JoVE Journal
Immunology and Infection
Nitrotetrazolium Blue Chloride (NBT) Assay for Evaluating ROS Production
Ensaio de cloreto de azul de nitrotetrazólio (NBT) para avaliação da produção de ROS
Neutrophil Functional Assays for Screening Different Signaling Pathways
DOI:
10.3791/200484-v
•
02:59 min
•
February 09, 2024
•
Isabelle Souza Luz
,
Raquel Takaya
,
Daiane Gonzaga Ribeiro
,
Natanael Sales Silva
,
Lucas Fontes
,
Mariana S. Castro
,
Wagner Fontes
1
Laboratory of Protein Chemistry and Biochemistry, Department of Cell Biology
,
University of Brasilia
,
2
Institute of Physics
,
University of Brasilia
Tags
NBT Assay
ROS Production
Polymorphonuclear Neutrophils
DMSO
HBSS
PMA
FMLP
NBT Slide Test
NBT Spectrophotometry Assay
Formazan Deposits
SDS
Sonication
Absorbance Measurement
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