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Journal
/
Biochemistry
/
Preparation of Electron Microscopy Grids for In Situ Cellular Cryotomography
/
Klargjøring av elektronmikroskopi (EM) for cellulær kryotomografi
JoVE Journal
Biochemistry
This content is Free Access.
JoVE Journal
Biochemistry
Preparing Electron Microscopy (EM) Grids for Cellular Cryotomography
Klargjøring av elektronmikroskopi (EM) for cellulær kryotomografi
Preparation of Electron Microscopy Grids for In Situ Cellular Cryotomography
DOI:
10.3791/200512-v
•
02:29 min
•
December 15, 2023
•
Noah Weber*
1,2
,
Brennan Hinks*
2,3
,
Jacob Jensen
,
Thomas Lidahl
3
,
Luiza Mendonça
1
College of Liberal Arts
,
University of Minnesota
,
2
Department of Biochemistry, Molecular Biology and Biophysics, Medical School
,
University of Minnesota
,
3
College of Biological Sciences
,
University of Minnesota
Tags
Keywords: Electron Microscopy
Cryotomography
EM Grids
Glow Discharge
Perforated Carbon Support Film
Bovine Fibronectin
Phosphate Buffered Saline
PBS
UV Sterilization
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