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Journal
/
Biochemistry
/
Preparation of Electron Microscopy Grids for In Situ Cellular Cryotomography
/
זריעת תאים והתמרה על רשתות מיקרוסקופ אלקטרונים (EM) לקריוטומוגרפיה
JoVE Journal
Biochemistry
This content is Free Access.
JoVE Journal
Biochemistry
Cell Seeding and Transfection on Electron Microscopy (EM) Grids for Cryotomography
Please note that all translations are automatically generated.
Click here for the English version.
זריעת תאים והתמרה על רשתות מיקרוסקופ אלקטרונים (EM) לקריוטומוגרפיה
Preparation of Electron Microscopy Grids for In Situ Cellular Cryotomography
DOI:
10.3791/200513-v
•
02:04 min
•
December 15, 2023
•
Noah Weber*
1,2
,
Brennan Hinks*
2,3
,
Jacob Jensen
,
Thomas Lidahl
3
,
Luiza Mendonça
1
College of Liberal Arts
,
University of Minnesota
,
2
Department of Biochemistry, Molecular Biology and Biophysics, Medical School
,
University of Minnesota
,
3
College of Biological Sciences
,
University of Minnesota
Tags
Keywords: Cell Seeding
Transfection
Electron Microscopy Grids
Cryotomography
HIV Molecular Clones
DMEM
Transfection Reagent
DNA
Cell Culture
Microscopy
Carbon Layer
Grid Squares
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