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Journal
/
Biochemistry
/
Preparation of Electron Microscopy Grids for In Situ Cellular Cryotomography
/
Congélation en plongée de grilles de microscopie électronique (EM) pour la cryotomographie
JoVE Journal
Biochemistry
This content is Free Access.
JoVE Journal
Biochemistry
Plunge Freezing of Electron Microscopy (EM) Grids for Cryotomography
Please note that all translations are automatically generated.
Click here for the English version.
Congélation en plongée de grilles de microscopie électronique (EM) pour la cryotomographie
Preparation of Electron Microscopy Grids for In Situ Cellular Cryotomography
DOI:
10.3791/200514-v
•
02:45 min
•
December 15, 2023
•
Noah Weber*
1,2
,
Brennan Hinks*
2,3
,
Jacob Jensen
,
Thomas Lidahl
3
,
Luiza Mendonça
1
College of Liberal Arts
,
University of Minnesota
,
2
Department of Biochemistry, Molecular Biology and Biophysics, Medical School
,
University of Minnesota
,
3
College of Biological Sciences
,
University of Minnesota
Tags
Keywords: Plunge Freezing
Electron Microscopy Grids
Cryotomography
Gold Fiducials
PBS
Blotting
Liquid Ethane
Cryo-correlative Light And Electron Microscopy
Cryo-electron Tomography
U2OS Cells
HIV Particles
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