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Journal
/
Biochemistry
/
Preparation of Electron Microscopy Grids for In Situ Cellular Cryotomography
/
냉동단층촬영을 위한 전자현미경(EM) 그리드의 플런지 동결
JoVE Journal
Biochemistry
This content is Free Access.
JoVE Journal
Biochemistry
Plunge Freezing of Electron Microscopy (EM) Grids for Cryotomography
Please note that all translations are automatically generated.
Click here for the English version.
냉동단층촬영을 위한 전자현미경(EM) 그리드의 플런지 동결
Preparation of Electron Microscopy Grids for In Situ Cellular Cryotomography
DOI:
10.3791/200514-v
•
02:45 min
•
December 15, 2023
•
Noah Weber*
1,2
,
Brennan Hinks*
2,3
,
Jacob Jensen
,
Thomas Lidahl
3
,
Luiza Mendonça
1
College of Liberal Arts
,
University of Minnesota
,
2
Department of Biochemistry, Molecular Biology and Biophysics, Medical School
,
University of Minnesota
,
3
College of Biological Sciences
,
University of Minnesota
Tags
Plunge Freezing
Electron Microscopy Grids
Cryotomography
Gold Fiducials
PBS
Blotting
Liquid Ethane
Cryo-correlative Light And Electron Microscopy
Cryo-electron Tomography
U2OS Cells
HIV Particles
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포유류 세포의
In Situ
Cryotomography를 위한 시료 전처리
세포 냉동 단층촬영을 위한 전자 현미경(EM) 그리드 준비
Cell seeding and transfection on Electron Microscopy (EM) Grids for Cryotomography(냉동단층촬영을 위한 전자 현미경(EM) 그리드의 세포 파종 및 형질주입(Cell seeding and transfection)
냉동단층촬영을 위한 전자현미경(EM) 그리드의 플런지 동결
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