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Journal
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Bioengineering
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Analysis of Blood-Brain Barrier Models via Immunofluorescence Staining
/
면역세포화학 염색 및 공초점 분석을 위한 μSiM 장치에서 혈액 뇌 장벽 단일 배양 확립
JoVE Journal
Bioengineering
This content is Free Access.
JoVE Journal
Bioengineering
Establishing Blood Brain Barrier Monoculture in µSiM Device for Immunocytochemistry Staining and Confocal Analysis
Please note that all translations are automatically generated.
Click here for the English version.
면역세포화학 염색 및 공초점 분석을 위한 μSiM 장치에서 혈액 뇌 장벽 단일 배양 확립
Analysis of Blood-Brain Barrier Models via Immunofluorescence Staining
DOI:
10.3791/200520-v
•
05:25 min
•
January 12, 2024
•
Molly C. McCloskey
,
Pelin Kasap
,
Michelle Trempel
,
Louis P. Widom
,
Julia Kuebel
,
Kaihua Chen
,
Thomas R. Gaborski
,
Britta Engelhardt
,
James L. McGrath
1
Department of Biomedical Engineering
,
University of Rochester
,
2
Theodor Kocher Institute
,
University of Bern
,
3
Department of Biomedical Engineering
,
Rochester Institute of Technology
Tags
Blood-brain Barrier
Monoculture
SiM Device
Immunocytochemistry
Confocal Analysis
Cell Culture
Collagen
Fibronectin
HECSR
EECM-BMEC
Cell Detachment
Endothelial Medium
Methanol Fixation
PBS Washing
Blocking
Primary Antibody
Secondary Antibody
Hoechst Staining
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check_url/200520?article_type=v
혈액-뇌 장벽 모델링을 위한 MicroSiM(μSiM) 장벽 조직 플랫폼 사용
check_url/200520?article_type=v
µSiM Device Assembly for Blood Brain Barrier Monoculture
check_url/200520?article_type=v
면역세포화학 염색 및 공초점 분석을 위한 μSiM 장치에서 혈액 뇌 장벽 단일 배양 확립
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