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Journal
/
Bioengineering
/
Analysis of Blood-Brain Barrier Models via Immunofluorescence Staining
/
Etablering av blodhjernebarrieremonokultur i μSiM-enhet for immuncytokjemisk farging og konfokalanalyse
JoVE Journal
Bioengineering
This content is Free Access.
JoVE Journal
Bioengineering
Establishing Blood Brain Barrier Monoculture in µSiM Device for Immunocytochemistry Staining and Confocal Analysis
Etablering av blodhjernebarrieremonokultur i μSiM-enhet for immuncytokjemisk farging og konfokalanalyse
Analysis of Blood-Brain Barrier Models via Immunofluorescence Staining
DOI:
10.3791/200520-v
•
05:25 min
•
January 12, 2024
•
Molly C. McCloskey
,
Pelin Kasap
,
Michelle Trempel
,
Louis P. Widom
,
Julia Kuebel
,
Kaihua Chen
,
Thomas R. Gaborski
,
Britta Engelhardt
,
James L. McGrath
1
Department of Biomedical Engineering
,
University of Rochester
,
2
Theodor Kocher Institute
,
University of Bern
,
3
Department of Biomedical Engineering
,
Rochester Institute of Technology
Tags
Blood-brain Barrier
Monoculture
SiM Device
Immunocytochemistry
Confocal Analysis
Cell Culture
Collagen
Fibronectin
HECSR
EECM-BMEC
Cell Detachment
Endothelial Medium
Methanol Fixation
PBS Washing
Blocking
Primary Antibody
Secondary Antibody
Hoechst Staining
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