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Journal
/
Neuroscience
/
Extraction and Enzymatic Purification of Microglia from the Mouse Spinal Cord
/
Purificação enzimática baseada em liberase de células microgliais isoladas de uma medula espinhal de camundongo
JoVE Journal
Neuroscience
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JoVE Journal
Neuroscience
Liberase-Based Enzymatic Purification of Microglial Cells Isolated from a Mouse Spinal Cord
Purificação enzimática baseada em liberase de células microgliais isoladas de uma medula espinhal de camundongo
Extraction and Enzymatic Purification of Microglia from the Mouse Spinal Cord
DOI:
10.3791/200616-v
•
02:42 min
•
September 22, 2023
•
Claudia I Gutiérrez-Román
2
,
María E Meléndez Camargo
,
Cuauhtémoc C García Rojas
,
Miguel Jimenez Olvera
,
Sandra H Gutiérrez Román
,
Oscar Medina-Contreras
1
Epidemiology, Endocrinology & Nutrition
,
Mexico Children’s Hospital
,
2
Pharmacy Department, National School of Biological Sciences
,
National Polytechnic Institute
,
3
Graduate Department
,
Dr. Eduardo Liceaga General Hospital of Mexico
,
4
Pain Clinic and Palliative Care
,
Dr. Eduardo Liceaga General Hospital of Mexico
,
5
General Surgery Service
,
Metropolitan Angeles Hospital
Tags
Microglia Isolation
Spinal Cord
Enzymatic Digestion
Liberase
Density Gradient
Cell Purification
FACS Analysis
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