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Journal
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Biology
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Enhanced Bacterial Extracellular Vesicles Isolation for Microbiota Multi-Omics
/
Etablering av et sentrifugeringssystem for tetthetsgradient for å isolere og rense bakterielle ekstracellulære vesikler (BEVs) fra menneskelig avføring
JoVE Journal
Biology
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JoVE Journal
Biology
Establishment of a Density Gradient Centrifugation System to Isolate and Purify Bacterial Extracellular Vesicles (BEVs) from Human Faeces
Etablering av et sentrifugeringssystem for tetthetsgradient for å isolere og rense bakterielle ekstracellulære vesikler (BEVs) fra menneskelig avføring
Enhanced Bacterial Extracellular Vesicles Isolation for Microbiota Multi-Omics
DOI:
10.3791/200625-v
•
03:07 min
•
September 01, 2023
•
Yicong Xue*
1
,
Xixin Huang*
1
,
Zihao Ou*
1
,
Yuanyuan Wu
,
Qianbei Li
,
Xinyue Huang
,
Minghui Wen
,
Yan Yang
,
Bo Situ
,
Lei Zheng
1
Department of Laboratory Medicine, Nanfang Hospital
,
Southern Medical University
Tags
Density Gradient Centrifugation
Bacterial Extracellular Vesicles (BEVs)
Isolation
Purification
Iodixanol Gradient
Ultracentrifugation
Fractionation
PBS
Human Feces
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Isolering og rensing av bakterielle ekstracellulære vesikler fra humane avføring ved bruk av tetthetsgradient sentrifugering
Berikelse av bakterielle ekstracellulære vesikler (BEV) fra human avføring ved bruk av differensielle sentrifugeringshastigheter
Etablering av et sentrifugeringssystem for tetthetsgradient for å isolere og rense bakterielle ekstracellulære vesikler (BEVs) fra menneskelig avføring
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