JoVE
JoVE
Faculty Resource Center
Research
Behavior
Biochemistry
Biology
Bioengineering
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
JoVE Journal
JoVE Encyclopedia of Experiments
JoVE Chrome Extension
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to your Course
Authors
Librarians
High Schools
About
Sign-In
Sign In
Contact Us
Research
JoVE Journal
JoVE Encyclopedia of Experiments
Education
JoVE Core
JoVE Science Education
JoVE Lab Manual
High Schools
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Close
Research
Behavior
Biochemistry
Bioengineering
Biology
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
Products
JoVE Journal
JoVE Encyclopedia of Experiments
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
Products
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to Your Course
Teacher Resources
Get in Touch
Instant Trial
Log In
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Journal
/
Bioengineering
/
Stable Gene Integration in
Chlorella vulgaris
Using
Agrobacterium tumefaciens
/
根癌农杆菌
的电穿孔用于微藻细胞的基因工程
JoVE Journal
Bioengineering
A subscription to JoVE is required to view this content.
Sign in or start your free trial.
JoVE Journal
Bioengineering
Electroporation of
Agrobacterium tumefaciens
for Genetic Engineering of Microalgal Cells
Please note that all translations are automatically generated.
Click here for the English version.
根癌农杆菌
的电穿孔用于微藻细胞的基因工程
Stable Gene Integration in
Chlorella vulgaris
Using
Agrobacterium tumefaciens
DOI:
10.3791/200637-v
•
01:16 min
•
October 27, 2023
•
M. Reza Roushan
,
Chuchi Chen
,
Parisa Ahmadi
,
Valerie C. A. Ward
1
Department of Chemical Engineering
,
University of Waterloo
Tags
Keywords: Electroporation
Agrobacterium Tumefaciens
Genetic Engineering
Microalgal Cells
PCAMBIA1302
Electroporator
LB Media
Antibiotic-supplemented LB Agar
Cryopreservation
Plasmid-containing Strain
Glycerol Solution
Embed
ADD TO PLAYLIST
Usage Statistics
Related Videos
check_url/200637?article_type=v
根癌农杆菌
介导的绿色微藻、
寻常小球藻
的基因工程
check_url/200637?article_type=v
用于微藻基因工程的电感受态
根癌农杆菌
培养物的制备
check_url/200637?article_type=v
根癌农杆菌
的电穿孔用于微藻细胞的基因工程
check_url/200637?article_type=v
农杆菌介导
的寻常小球藻
转化及通过菌落PCR（cPCR）确认基因整合
Read Article
