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Biology
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Quantitative Analysis of Mitophagy in Murine Pancreatic β-Cells
/
Одноклеточная диссоциация мышиных островков для проточного цитометрического анализа β-клеточной митофагии
JoVE Journal
Biology
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JoVE Journal
Biology
Single-Cell Dissociation of Murine Islets for Flow Cytometric Analysis of β-Cell Mitophagy
Please note that all translations are automatically generated.
Click here for the English version.
Одноклеточная диссоциация мышиных островков для проточного цитометрического анализа β-клеточной митофагии
Quantitative Analysis of Mitophagy in Murine Pancreatic β-Cells
DOI:
10.3791/200660-v
•
01:43 min
•
September 15, 2023
•
Elena Levi-D’Ancona
2
,
Vaibhav Sidarala
,
Scott A. Soleimanpour
3,4
1
Department of Internal Medicine, Division of Metabolism, Endocrinology and Diabetes
,
University of Michigan, Ann Arbor
,
2
Graduate Program in Immunology
,
University of Michigan Medical School
,
3
Department of Molecular and Integrative Physiology
,
University of Michigan, Ann Arbor
,
4
VA Ann Arbor Healthcare System
Tags
Keywords: Single-cell Dissociation
Murine Islets
Flow Cytometric Analysis
β-cell Mitophagy
Islet Culture
High-fat Diet
Valinomycin
Trypsin
Cell Staining
Flow Cytometry
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