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Análise por Citometria de Fluxo da Mitofagia em Células β Pancreáticas Murinas Utilizando o Corante MtPhagy
JoVE Journal
Biology
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JoVE Journal
Biology
Flow Cytometric Analysis of Mitophagy in Murine Pancreatic β-Cells Using MtPhagy Dye
Análise por Citometria de Fluxo da Mitofagia em Células β Pancreáticas Murinas Utilizando o Corante MtPhagy
DOI:
10.3791/200661-v
•
02:37 min
•
September 15, 2023
•
Elena Levi-D’Ancona
2
,
Vaibhav Sidarala
,
Scott A. Soleimanpour
3,4
1
Department of Internal Medicine, Division of Metabolism, Endocrinology and Diabetes
,
University of Michigan, Ann Arbor
,
2
Graduate Program in Immunology
,
University of Michigan Medical School
,
3
Department of Molecular and Integrative Physiology
,
University of Michigan, Ann Arbor
,
4
VA Ann Arbor Healthcare System
Tags
Flow Cytometry
Mitophagy
Pancreatic Beta Cells
MtPhagy Dye
TMRE
Fluozin-3-AM
DAPI
Cell Suspension
Islet Isolation
Fluorescence Gating
Quantification
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