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Journal
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Biology
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Quantitative Analysis of Mitophagy in Murine Pancreatic β-Cells
/
マウス膵臓β細胞における遺伝的にコードされたmt-Keima Reporterを用いたマイトファジーのフローサイトメトリー解析
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Biology
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JoVE Journal
Biology
Flow Cytometric Analysis of Mitophagy in Murine Pancreatic β-Cells Using the Genetically Encoded mt-Keima Reporter
Please note that all translations are automatically generated.
Click here for the English version.
マウス膵臓β細胞における遺伝的にコードされたmt-Keima Reporterを用いたマイトファジーのフローサイトメトリー解析
Quantitative Analysis of Mitophagy in Murine Pancreatic β-Cells
DOI:
10.3791/200662-v
•
01:45 min
•
September 15, 2023
•
Elena Levi-D’Ancona
2
,
Vaibhav Sidarala
,
Scott A. Soleimanpour
3,4
1
Department of Internal Medicine, Division of Metabolism, Endocrinology and Diabetes
,
University of Michigan, Ann Arbor
,
2
Graduate Program in Immunology
,
University of Michigan Medical School
,
3
Department of Molecular and Integrative Physiology
,
University of Michigan, Ann Arbor
,
4
VA Ann Arbor Healthcare System
Tags
Flow Cytometry
Mitophagy
Pancreatic Beta Cells
Mt-Keima Reporter
FluoZin-3-AM
DAPI
Single Cell Analysis
Gating Strategy
Valinomycin
Mitophagy Flux
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膵β細胞におけるマイトファジーフラックスを調べるための補完的アプローチ
β細胞マイトファジーのフローサイトメトリー解析のためのマウス膵島の単一細胞解離
MtPhagy 色素を用いたマウス膵臓β細胞におけるマイトファジーのフローサイトメトリー解析
マウス膵臓β細胞における遺伝的にコードされたmt-Keima Reporterを用いたマイトファジーのフローサイトメトリー解析
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