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A 3D Blood-Brain Barrier Model Derived from Human Pluripotent Stem-Cells

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Preparación de células para ensamblar una barrera hematoencefálica in vitro derivada de células madre humanas en 3D

JoVE Journal
Neuroscience

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JoVE Journal Neuroscience

Preparing Cells to Assemble a 3D Human-Stem-Cell-Derived In Vitro Blood Brain Barrier

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Preparación de células para ensamblar una barrera hematoencefálica in vitro derivada de células madre humanas en 3D

A 3D Blood-Brain Barrier Model Derived from Human Pluripotent Stem-Cells

DOI:

10.3791/200667-v

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02:05 min

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October 20, 2023

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Camille Goldman*^1,2,3,4,5, Natalie Suhy*^1,2,3,4,5, Jessica E. Schwarz^2,3,4,5, Emily Ruth Sartori^2,3,4,5, Rikki B. Rooklin^2,3,4,5, Braxton R. Schuldt^2,3,4,5, Louise A. Mesentier-Louro^2,3,4,5, Joel W. Blanchard^2,3,4,5

¹Icahn School of Medicine at Mount Sinai, ²Black Family Stem Cell Institute at Mount Sinai, ³Ronald M. Loeb Center for Alzheimer’s Disease at Mount Sinai, ⁴Friedman Brain Institute at Mount Sinai, ⁵Nash Family Department of Neuroscience at Mount Sinai

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3D Human-stem-cell-derived In Vitro Blood Brain Barrier Differentiated Endothelial Cells Protease Collagenase Dissociation HECSR Differentiated Parasites N2B27 Differentiated Astrocytes Complete Astrocyte Medium IBBB Assembly

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