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Journal
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Biochemistry
/
Strategies for Expressing and Purifying Solute Carrier Transporters
/
उच्च-थ्रूपुट प्रोटीन अभिव्यक्ति के लिए pHTBV1.1 Bacmid में विलेय वाहक (SLCs) की क्लोनिंग
JoVE Journal
Biochemistry
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JoVE Journal
Biochemistry
Cloning of Solute Carriers (SLCs) into pHTBV1.1 Bacmid for High-Throughput Protein Expression
Please note that all translations are automatically generated.
Click here for the English version.
उच्च-थ्रूपुट प्रोटीन अभिव्यक्ति के लिए pHTBV1.1 Bacmid में विलेय वाहक (SLCs) की क्लोनिंग
Strategies for Expressing and Purifying Solute Carrier Transporters
DOI:
10.3791/200669-v
•
01:12 min
•
September 29, 2023
•
Sagar Raturi
,
Huanyu Li
,
Yung-Ning Chang
,
Andreea Scacioc
,
Tina Bohstedt
,
Alejandra Fernandez-Cid
,
Adam Evans
,
Patrizia Abrusci
,
Abilasha Balakrishnan
,
Tomas C. Pascoa
,
Didi He
,
Gamma Chi
,
Nanki Kaur Singh
,
Mingda Ye
,
Anna Li
,
Leela Shrestha
,
Dong Wang
,
Eleanor P. Williams
,
Nicola A. Burgess-Brown
,
Katharina L. Dürr
,
Vera Puetter
,
Alvaro Ingles-Prieto
,
David B. Sauer
1
Centre for Medicines Discovery, Nuffield Department of Medicine
,
University of Oxford
,
2
Nuvisan ICB GmbH
,
3
CeMM Research Center for Molecular Medicine of the Austrian Academy of Sciences
Tags
SLC Clones
PHTBV1.1 Bacmid
Protein Expression
Cloning
Recombinant Enzyme
Proteinase K
Chemically Competent E.coli Mach1
Heat Shock Transformation
Colony PCR
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उच्च-थ्रूपुट प्रोटीन अभिव्यक्ति के लिए pHTBV1.1 Bacmid में विलेय वाहक (SLCs) की क्लोनिंग
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