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Journal
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Biology
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High-Quality Sample Preparation for Multiome 10X Genomics Analysis
/
Purificazione di nuclei da cellule staminali e progenitrici ematopoietiche del midollo osseo di topo (HSPC)
JoVE Journal
Biology
This content is Free Access.
JoVE Journal
Biology
Purification of Nuclei from Mouse Bone Marrow Hematopoietic Stem and Progenitor Cells (HSPCs)
Please note that all translations are automatically generated.
Click here for the English version.
Purificazione di nuclei da cellule staminali e progenitrici ematopoietiche del midollo osseo di topo (HSPC)
High-Quality Sample Preparation for Multiome 10X Genomics Analysis
DOI:
10.3791/200693-v
•
03:48 min
•
December 22, 2023
•
Carolina Moraes Cabé
,
Sophie Novault
,
Ana Jeemin Choi
,
Valerie Seffer
,
Laura Barrio Cano
,
Valentina Libri
3
,
Milena Hasan
1
Cytometry and Biomarkers UTechS, C2RT
,
Institut Pasteur, Université Paris Cité
,
2
Microenvironment and Immunity Unit
,
Institut Pasteur, Université Paris Cité, INSERM U1224
,
3
Istituto Superiore di Sanità
Tags
Keywords: Nuclei Purification
Mouse Bone Marrow
Hematopoietic Stem And Progenitor Cells (HSPCs)
ACK Lysing Buffer
Cell Strainer
FACS Buffer
Cell Counting
Cell Staining
Flow Cytometry
Cell Sorting
Collection Medium
PBS
BSA
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