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Journal
/
Biochemistry
/
Analyzing Mitochondrial Physiology and Bioenergetics in Fruit Flies
/
Substrate-uncoupler-inhibitor-titration for mitochondrial respiration analysis in
Drosophila melanogaster
JoVE Journal
Biochemistry
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JoVE Journal
Biochemistry
Substrate-Uncoupler-Inhibitor-Titration for Mitochondrial Respiration Analysis in
Drosophila melanogaster
Please note that all translations are automatically generated.
Click here for the English version.
Substrate-uncoupler-inhibitor-titration for mitochondrial respiration analysis in
Drosophila melanogaster
Analyzing Mitochondrial Physiology and Bioenergetics in Fruit Flies
DOI:
10.3791/200718-v
•
05:12 min
•
November 10, 2023
•
Paula Michelotti*
1,2
,
Tâmie Duarte*
1,2
,
Cristiane L. Dalla Corte
2
1
Laboratory of Experimental Biochemistry and Toxicology, Department of Biochemistry and Molecular Biology, Center of Natural and Exact Sciences
,
Federal University of Santa Maria
,
2
Graduate Program in Biological Sciences: Toxicological Biochemistry
,
Federal University of Santa Maria
Tags
Mitochondrial Respiration
Drosophila Melanogaster
Substrate
Uncoupler
Inhibitor
Titration
Polarographic Oxygen Sensors
MiR05 Buffer
High-resolution Respirometry
Digitonin
Pyruvate
Malate
Proline
ADP
Succinate
Oligomycin
FCCP
Rotenone
Malonate
Antimycin
OXPHOS CI
OXPHOS CII
ETS CI
ETS CII
ATP Synthesis
OXPHOS/LEAK Ratio
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