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Journal
/
Biochemistry
/
Crystallization of ABC Transporter Membrane Proteins in Lipidic Bicelles
/
Reduktive Methylierungs- und Vorkristallisationsbehandlung von gereinigtem heterodimerem ABCG5/G8-Protein
JoVE Journal
Biochemistry
This content is Free Access.
JoVE Journal
Biochemistry
Reductive Methylation and Pre-Crystallization Treatment of Purified Heterodimeric ABCG5/G8 Protein
Please note that all translations are automatically generated.
Click here for the English version.
Reduktive Methylierungs- und Vorkristallisationsbehandlung von gereinigtem heterodimerem ABCG5/G8-Protein
Crystallization of ABC Transporter Membrane Proteins in Lipidic Bicelles
DOI:
10.3791/200736-v
•
02:35 min
•
August 25, 2023
•
Sarah Wazir
,
Danny Farhat
,
Mahalashmi Srinivasan
2
,
Jyh-Yeuan Lee
1
Department of Biochemistry, Microbiology and Immunology, Faculty of Medicine
,
University of Ottawa
,
2
Department of Biochemistry, Faculty of Medicine and Dentistry
,
University of Alberta
Tags
Keywords: Reductive Methylation
ABCG5/G8 Protein
Purification
Pre-crystallization Treatment
Nickel-NTA Column
PD-10 Desalting
Cholesterol Addition
Ultracentrifugation
Protein Concentration
Analytical Gel Filtration
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