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Journal
/
Biochemistry
/
Crystallization of ABC Transporter Membrane Proteins in Lipidic Bicelles
/
Krystallisering av heterodimert ABCG5/G8-protein i lipidbicelle ved bruk av hengende dråpedampdiffusjon
JoVE Journal
Biochemistry
This content is Free Access.
JoVE Journal
Biochemistry
Crystallization of Heterodimeric ABCG5/G8 Protein in Lipidic Bicelle Using Hanging Drop Vapor Diffusion
Krystallisering av heterodimert ABCG5/G8-protein i lipidbicelle ved bruk av hengende dråpedampdiffusjon
Crystallization of ABC Transporter Membrane Proteins in Lipidic Bicelles
DOI:
10.3791/200737-v
•
02:21 min
•
August 25, 2023
•
Sarah Wazir
,
Danny Farhat
,
Mahalashmi Srinivasan
2
,
Jyh-Yeuan Lee
1
Department of Biochemistry, Microbiology and Immunology, Faculty of Medicine
,
University of Ottawa
,
2
Department of Biochemistry, Faculty of Medicine and Dentistry
,
University of Alberta
Tags
Keywords: Crystallization
Heterodimeric
ABCG5/G8 Protein
Lipidic Bicelle
Hanging Drop Vapor Diffusion
Bicelle Stock Solution
DMPC
CHAPSO
Protein-bicelle Mixture
Crystallization Reservoir Solution
Crystal Growth
Sodium Malate
Cryo Loops
Data Collection
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