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Journal
/
Developmental Biology
/
Investigating Development with Optogenetic Signaling Manipulation in Zebrafish
/
הזרקת מבנים bOpto-BMP/nodal לעוברים של דגי זברה
JoVE Journal
Developmental Biology
This content is Free Access.
JoVE Journal
Developmental Biology
Injecting bOpto-BMP/Nodal Constructs into Zebrafish Embryos
Please note that all translations are automatically generated.
Click here for the English version.
הזרקת מבנים bOpto-BMP/nodal לעוברים של דגי זברה
Investigating Development with Optogenetic Signaling Manipulation in Zebrafish
DOI:
10.3791/200757-v
•
01:56 min
•
October 27, 2023
•
Allison J. Saul*
1
,
Catherine E. Rogers*
1
,
Marcial Garmendia-Cedillos
,
Thomas Pohida
,
Katherine W. Rogers
1
Division of Developmental Biology, Eunice Kennedy Shriver National Institute of Child Health and Human Development
,
NIH
,
2
Instrumentation Development and Engineering Application Solutions, National Institute of Biomedical Imaging and Bioengineering
,
NIH
Tags
Zebrafish Embryos
Opto-BMP-Nodal Constructs
Light Box
LED Microplate Illuminator
Injection Dishes
Agarose-coated Plates
Glass Pipettes
Immunofluorescence
Phenotyping Assay
Developmental Stages
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הפעלת איתות אופטוגנטי בעוברים של דגי זברה
הזרקת מבנים bOpto-BMP/nodal לעוברים של דגי זברה
ניסוי חשיפה לאור להפעלת איתות BMP או Nodal בדגי זברה
הערכה של עוברים המבטאים bOpto-BMP/Nodal לאחר ניסוי החשיפה לאור
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