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Journal
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Cancer Research
/
Evaluating Quantitative Tumor Cell Killing by CAR-Expressing Jurkat Cells
/
CFSE 표지 종양 세포를 사용한 CAR-Jurkat 공동 배양에서 형광 이미징 및 세포 생존율 평가를 위한 플레이트 준비
JoVE Journal
Cancer Research
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JoVE Journal
Cancer Research
Preparation of Plate for Fluorescent Imaging and Cell Viability Assessment in CAR-Jurkat Co-Culture with CFSE-Labeled Tumor Cells
Please note that all translations are automatically generated.
Click here for the English version.
CFSE 표지 종양 세포를 사용한 CAR-Jurkat 공동 배양에서 형광 이미징 및 세포 생존율 평가를 위한 플레이트 준비
Evaluating Quantitative Tumor Cell Killing by CAR-Expressing Jurkat Cells
DOI:
10.3791/200793-v
•
04:00 min
•
October 27, 2023
•
Siddharth Subham*
1,2,3
,
John D. Jeppson*
1
,
Benjamin K. Gibbs
,
Jacqueline Babai
,
Riza Alker
,
Andrew K. Godwin
5,6
,
David Akhavan
2,3
1
Department of Radiation Oncology
,
University of Kansas Cancer Center
,
2
Department of Cancer Biology
,
University of Kansas Cancer Center
,
3
BioEngineering Program
,
University of Kansas
,
4
Department of Pathology and Laboratory Medicine
,
University of Kansas Medical Center
,
5
University of Kansas Cancer Center
,
6
Kansas Institute for Precision Medicine
,
University of Kansas Medical Center
Tags
Fluorescent Imaging
Cell Viability
CAR-Jurkat Co-culture
CFSE-labeled Tumor Cells
Propidium Iodide
Triton X
Cell Imaging
CFSE Fluorescence
Cell Diameter
Live/dead Cell Analysis
Cell Counting
Effector-to-target Ratio
EGFR-targeting CAR
Cytotoxicity
PBMC-derived CD3 T Cells
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check_url/200793?article_type=v
CFSE 표지 종양 세포를 사용한 CAR-Jurkat 공동 배양에서 형광 이미징 및 세포 생존율 평가를 위한 플레이트 준비
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