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Journal
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Biology
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Efficient Rat Bone Marrow Neutrophil Extracellular Trap Isolation
/
En og to-trinns metode for nøytrofil isolasjon fra rotter for høsting av nøytrofile ekstracellulære feller (NET)
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JoVE Journal
Biology
One and Two-Step Method of Rat Neutrophil Isolation for Harvesting Neutrophil Extracellular Traps (NETs)
En og to-trinns metode for nøytrofil isolasjon fra rotter for høsting av nøytrofile ekstracellulære feller (NET)
Efficient Rat Bone Marrow Neutrophil Extracellular Trap Isolation
DOI:
10.3791/200810-v
•
03:01 min
•
April 26, 2024
•
Xiangfeng Gong*
1
,
Yutao Sun*
1
,
Xiaoxin Zhang
3
,
Zhenghua Xiao
4
,
Li He
4
,
Chaoyi Qin
4
1
Department of Cardiovascular Surgery, West China Hospital
,
Sichuan University
,
2
Department of Integrated Traditional Chinese and Western Medicine, Sichuan Provincial Pancreatitis Centre, West China Hospital
,
Sichuan University
,
3
West China-Liverpool Biomedical Research Centre, West China Hospital
,
Sichuan University
,
4
Cardiovascular Surgery Research Laboratory, West China Hospital
,
Sichuan University
Tags
Rat Neutrophil Isolation
Neutrophil Extracellular Traps (NETs)
Bone Marrow Harvest
Density Gradient Centrifugation
One-step Method
Two-step Method
RPMI Media
Red Blood Cell Lysis Buffer
Percoll Reagent
HBSS
Cell Viability
Hemocytometer
Trypan Blue Staining
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