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Efficient Rat Bone Marrow Neutrophil Extracellular Trap Isolation

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大鼠中性粒细胞胞外陷阱（NET）的荧光定量和细胞分析

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Biology

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JoVE Journal Biology

Fluorometric Quantification and Cytometric Analysis of Rat Neutrophil Extracellular Traps (NETs)

Please note that all translations are automatically generated. Click here for the English version.

大鼠中性粒细胞胞外陷阱（NET）的荧光定量和细胞分析

Efficient Rat Bone Marrow Neutrophil Extracellular Trap Isolation

DOI:

10.3791/200812-v

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02:06 min

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April 26, 2024

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Xiangfeng Gong*¹, Yutao Sun*¹, Xiaoxin Zhang³, Zhenghua Xiao⁴, Li He⁴, Chaoyi Qin⁴

¹Department of Cardiovascular Surgery, West China Hospital,Sichuan University, ²Department of Integrated Traditional Chinese and Western Medicine, Sichuan Provincial Pancreatitis Centre, West China Hospital,Sichuan University, ³West China-Liverpool Biomedical Research Centre, West China Hospital,Sichuan University, ⁴Cardiovascular Surgery Research Laboratory, West China Hospital,Sichuan University

Tags

Fluorometric Quantification Cytometric Analysis Rat Neutrophil Extracellular Traps (NETs)Tris-EDTA Buffer DNA Standards Assay Reagent Fluorescence Microplate Reader Nucleus Stain Cell-free DNA Stain Cytometer SYTOX Green HEXT Channel PMA Stimulation Cell-free DNA Content

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