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Journal
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Developmental Biology
/
Processing Gut Cells from Zebrafish Larvae for Single-Cell RNA Sequencing
/
야생형 및 형질전환 제브라피시 유충 및 WildType 전체 유충 해리 획득
JoVE Journal
Developmental Biology
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JoVE Journal
Developmental Biology
Obtaining Wildtype and Transgenic Zebrafish Larvae and WildType Whole Larvae Dissociation
Please note that all translations are automatically generated.
Click here for the English version.
야생형 및 형질전환 제브라피시 유충 및 WildType 전체 유충 해리 획득
Processing Gut Cells from Zebrafish Larvae for Single-Cell RNA Sequencing
DOI:
10.3791/200859-v
•
01:30 min
•
November 10, 2023
•
Naomi J. M. Kakiailatu
,
Laura E. Kuil
2
,
Eric Bindels
,
Joke T. M. Zink
,
Michael Vermeulen
,
Veerle Melotte
,
Maria M. Alves
1
Department of Clinical Genetics
,
Erasmus University Medical Center – Sophia Children’s Hospital
,
2
Division of Psychosocial Research and Epidemiology
,
the Netherlands Cancer Institute
,
3
Department of Hematology
,
Erasmus Medical Center
,
4
Department of Pathology, GROW-School for Oncology and Developmental Biology
,
Maastricht University Medical Center
Tags
Keywords: Zebrafish
Wildtype
Transgenic
Larvae
Dissociation
HEPES-buffered E3 Medium
Trypsin-EDTA
Anesthesia
Tricaine
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