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Processing Gut Cells from Zebrafish Larvae for Single-Cell RNA Sequencing
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Isolement et dissociation de l’intestin des larves de poisson-zèbre pour l’enrichissement des cellules intestinales par FACS
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Developmental Biology
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JoVE Journal Developmental Biology
Isolation and Dissociation of Gut from Zebrafish Larvae for FACS Enrichment of Gut Cells
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Isolement et dissociation de l’intestin des larves de poisson-zèbre pour l’enrichissement des cellules intestinales par FACS

Processing Gut Cells from Zebrafish Larvae for Single-Cell RNA Sequencing

DOI:
10.3791/200860-v

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02:37 min

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November 10, 2023

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, , , , , ,
1Department of Clinical Genetics,Erasmus University Medical Center – Sophia Children’s Hospital, 2Division of Psychosocial Research and Epidemiology,the Netherlands Cancer Institute, 3Department of Hematology,Erasmus Medical Center, 4Department of Pathology, GROW-School for Oncology and Developmental Biology,Maastricht University Medical Center

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GutZebrafish LarvaeFACSCell IsolationDissociationPapainAgarose PlateHEPESE3 MediumDissectionIntestineCell StrainerCentrifugationDAPICell Labeling

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