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Journal
/
Bioengineering
/
Streamlined Protocol for Mouse CAR-T Cell Generation in Syngeneic Models
/
Transfecção e Transdução de Células T Murinas para Geração de Células CAR-T
JoVE Journal
Bioengineering
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JoVE Journal
Bioengineering
Transfection and Transduction of Murine T-Cells for CAR-T Cell Generation
Transfecção e Transdução de Células T Murinas para Geração de Células CAR-T
Streamlined Protocol for Mouse CAR-T Cell Generation in Syngeneic Models
DOI:
10.3791/200881-v
•
05:34 min
•
February 02, 2024
•
Rosa L. Vincent
,
Fangda Li
,
Edward R. Ballister
,
Nicholas Arpaia
3
,
Tal Danino
3,4
1
Department of Biomedical Engineering
,
Columbia University
,
2
Department of Microbiology & Immunology, Vagelos College of Physicians and Surgeons
,
Columbia University
,
3
Herbert Irving Comprehensive Cancer Center
,
Columbia University
,
4
Data Science Institute
,
Columbia University
Tags
Keywords: Murine T-cells
CAR-T Cell Generation
Transfection
Transduction
Phoenix Eco Plate
Viral Supernatant
Splenocytes
CD3 Positive T-cells
Negative Selection
T-cell Activation
Human Fibronectin Transduction Enhancer
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