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Journal
/
Developmental Biology
/
Detection and Quantification of Single mRNA Molecules in
Drosophila
/
Dissezione e preparazione di dischi alari e muscoli adulti da
Drosophila
per l'ibridazione in situ a fluorescenza di RNA a singola molecola
JoVE Journal
Developmental Biology
This content is Free Access.
JoVE Journal
Developmental Biology
Dissection and Preparation of Wing Discs and Adult Muscles from
Drosophila
for Single-Molecule RNA Fluorescence In Situ Hybridization
Please note that all translations are automatically generated.
Click here for the English version.
Dissezione e preparazione di dischi alari e muscoli adulti da
Drosophila
per l'ibridazione in situ a fluorescenza di RNA a singola molecola
Detection and Quantification of Single mRNA Molecules in
Drosophila
DOI:
10.3791/200894-v
•
03:46 min
•
September 08, 2023
•
Emma Leroux
,
Nourhene Ammar
,
Savannah Moinet
,
Thierry Pecot
,
Hadi Boukhatmi
1
Institut de Génétique et Développement de Rennes (IGDR)
,
UMR6290 CNRS – Université de Rennes
,
2
BIOSIT, UAR 3480 US 018
,
Université de Rennes
Tags
Drosophila
Wing Discs
Adult Muscles
Single-molecule RNA FISH
Dissection
Paraformaldehyde
Ethanol
PBS
PBT
Hybridization
Probe
Antibody
DAPI
Mounting Medium
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