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Journal
/
Developmental Biology
/
Detection and Quantification of Single mRNA Molecules in
Drosophila
/
Imagerie confocale et analyse quantitative de smFISH dans les disques alaires et les muscles adultes
JoVE Journal
Developmental Biology
This content is Free Access.
JoVE Journal
Developmental Biology
Confocal Imaging and Quantitative Analysis of smFISH in Wing Discs and Adult Muscles
Please note that all translations are automatically generated.
Click here for the English version.
Imagerie confocale et analyse quantitative de smFISH dans les disques alaires et les muscles adultes
Detection and Quantification of Single mRNA Molecules in
Drosophila
DOI:
10.3791/200895-v
•
04:16 min
•
September 08, 2023
•
Emma Leroux
,
Nourhene Ammar
,
Savannah Moinet
,
Thierry Pecot
,
Hadi Boukhatmi
1
Institut de Génétique et Développement de Rennes (IGDR)
,
UMR6290 CNRS – Université de Rennes
,
2
BIOSIT, UAR 3480 US 018
,
Université de Rennes
Tags
Keywords: Confocal Imaging
SmFISH
Wing Discs
Adult Muscles
DAPI
Alexa Fluor 488
Quasar 670
Image J
Mef2
Zfh1
Transcription Sites
MRNA
Quantitative Analysis
AMP
IFM
Image J Macro
Nuclei Segmentation
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