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Journal
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Genetics
/
A Guide to Precise Gene Disruption in Primary Bone Marrow-Derived Macrophages
/
Preparation for Electroporation and Ribonucleoprotein Delivery for Genome Editing in Mouse Bone Marrow-Derived Macrophages
JoVE Journal
Genetics
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JoVE Journal
Genetics
Preparation for Electroporation and Ribonucleoprotein Delivery for Genome Editing in Mouse Bone Marrow-Derived Macrophages
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Click here for the English version.
Preparation for Electroporation and Ribonucleoprotein Delivery for Genome Editing in Mouse Bone Marrow-Derived Macrophages
A Guide to Precise Gene Disruption in Primary Bone Marrow-Derived Macrophages
DOI:
10.3791/200896-v
•
04:03 min
•
August 04, 2023
•
Julia Craft
,
Tina Truong
,
Bennett H. Penn
2
1
Department of Internal Medicine
,
University of California, Davis
,
2
Department of Medical Microbiology and Immunology
,
University of California, Davis
Tags
Electroporation
Ribonucleoprotein
Genome Editing
Mouse Bone Marrow-derived Macrophages
EDTA
PBS
Cell Count
Cas9
SgRNA
Ribonucleoprotein Complex
Cell Suspension
Electroporation Tip
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