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A Guide to Precise Gene Disruption in Primary Bone Marrow-Derived Macrophages
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Preparation for Electroporation and Ribonucleoprotein Delivery for Genome Editing in Mouse Bone Marrow-Derived Macrophages
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Genetics
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JoVE Journal Genetics
Preparation for Electroporation and Ribonucleoprotein Delivery for Genome Editing in Mouse Bone Marrow-Derived Macrophages
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Preparation for Electroporation and Ribonucleoprotein Delivery for Genome Editing in Mouse Bone Marrow-Derived Macrophages

A Guide to Precise Gene Disruption in Primary Bone Marrow-Derived Macrophages

DOI:
10.3791/200896-v

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04:03 min

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August 04, 2023

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, ,
1Department of Internal Medicine,University of California, Davis, 2Department of Medical Microbiology and Immunology,University of California, Davis

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Keywords: ElectroporationRibonucleoproteinGenome EditingMouse Bone Marrow-derived MacrophagesEDTAPBSCell CountCas9SgRNARibonucleoprotein ComplexCell SuspensionElectroporation Tip

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