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Revealing Murine Pulmonary Veins Myocardial Architecture

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Inclusione tissutale e colorazione con immunofluorescenza di manicotti miocardici nelle vene polmonari murine

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Medicine

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JoVE Journal Medicine

Tissue Embedding and Immunofluorescence Staining of Myocardial Sleeves in Murine Pulmonary Veins

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Inclusione tissutale e colorazione con immunofluorescenza di manicotti miocardici nelle vene polmonari murine

Revealing Murine Pulmonary Veins Myocardial Architecture

DOI:

10.3791/200917-v

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04:14 min

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November 21, 2023

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Hannes E. Villgrater*^1,2,3, Ruibing Xia*^1,2,3, Aparna Sharma Chivukula^2,3, Philipp Tomsits*^1,2,3, Sebastian Clauss*^1,2,3,4

¹University Hospital Munich, Department of Medicine I,Ludwig-Maximilians-University Munich (LMU), ²Walter Brendel Center of Experimental Medicine (WBex),LMU Munich, ³German Center for Cardiovascular Research (DZHK), Partner Site Munich, Munich Heart Alliance (MHA), ⁴Interfaculty Center for Endocrine and Cardiovascular Disease Network Modelling and Clinical Transfer (ICONLMU),LMU Munich

Tags

Immunofluorescence Staining Myocardial Sleeves Murine Pulmonary Veins Cryomold OCT Compound Paraformaldehyde PBS Permeabilization Blocking Buffer Primary Antibody Secondary Antibody Hoechst 33342 Fluorescent Mounting Medium

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