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Journal
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Medicine
/
Revealing Murine Pulmonary Veins Myocardial Architecture
/
Incorporação tecidual e coloração por imunofluorescência de mangas miocárdicas em veias pulmonares murinas
JoVE Journal
Medicine
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JoVE Journal
Medicine
Tissue Embedding and Immunofluorescence Staining of Myocardial Sleeves in Murine Pulmonary Veins
Incorporação tecidual e coloração por imunofluorescência de mangas miocárdicas em veias pulmonares murinas
Revealing Murine Pulmonary Veins Myocardial Architecture
DOI:
10.3791/200917-v
•
04:14 min
•
November 21, 2023
•
Hannes E. Villgrater*
1,2,3
,
Ruibing Xia*
1,2,3
,
Aparna Sharma Chivukula
2,3
,
Philipp Tomsits*
1,2,3
,
Sebastian Clauss*
1,2,3,4
1
University Hospital Munich, Department of Medicine I
,
Ludwig-Maximilians-University Munich (LMU)
,
2
Walter Brendel Center of Experimental Medicine (WBex)
,
LMU Munich
,
3
German Center for Cardiovascular Research (DZHK), Partner Site Munich, Munich Heart Alliance (MHA)
,
4
Interfaculty Center for Endocrine and Cardiovascular Disease Network Modelling and Clinical Transfer (ICONLMU)
,
LMU Munich
Tags
Immunofluorescence Staining
Myocardial Sleeves
Murine Pulmonary Veins
Cryomold
OCT Compound
Paraformaldehyde
PBS
Permeabilization
Blocking Buffer
Primary Antibody
Secondary Antibody
Hoechst 33342
Fluorescent Mounting Medium
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