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Neuroscience
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An In Situ Immunostaining Protocol for Parkinson's Disease Study
/
Анализ морфологии митохондрий с помощью иммуногистохимии на срезах мозга, встроенных в парафин, от мышей, которым вводили α-синуклеин предварительно сформированные фибриллы (PFF)
JoVE Journal
Neuroscience
This content is Free Access.
JoVE Journal
Neuroscience
Mitochondrial Morphology Analysis by Immunohistochemistry on Paraffin-Embedded Brain Slices from α-Synuclein Pre-Formed Fibrils (PFF)-Injected Mice
Please note that all translations are automatically generated.
Click here for the English version.
Анализ морфологии митохондрий с помощью иммуногистохимии на срезах мозга, встроенных в парафин, от мышей, которым вводили α-синуклеин предварительно сформированные фибриллы (PFF)
An In Situ Immunostaining Protocol for Parkinson's Disease Study
DOI:
10.3791/201001-v
•
04:21 min
•
June 23, 2023
•
Dalila Ciceri*
1
,
Lierni Gregorio-Zabala*
1
,
Xabier Llama-Pino
,
Begüm Kurt
,
Jon Olano-Bringas
,
Patricia Villegas-Zafra
,
Nora Bengoa-Vergniory
2,3,4
1
Achucarro Basque Center for Neuroscience
,
2
Department of Neuroscience
,
University of the Basque Country (UPV/EHU)
,
3
Ikerbasque – Basque Foundation for Science
,
4
Oxford Parkinson’s Disease Centre and Department of Physiology, Anatomy and Genetics
,
University of Oxford
Tags
Mitochondrial Morphology
Immunohistochemistry
Paraffin-embedded
Brain Slices
α-synuclein
Pre-formed Fibrils (PFF)
C57 Black Six Mice
Citrate Buffer
TBS-T
Blocking
Primary Antibody
Secondary Antibody
DAPI
Mounting Reagent
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Histological Examination of Mitochondrial Morphology in a Parkinson's Disease Model
Анализ морфологии митохондрий с помощью иммуногистохимии на срезах мозга, встроенных в парафин, от мышей, которым вводили α-синуклеин предварительно сформированные фибриллы (PFF)
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