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Centromere-Associated Protein-E Gene Editing Using the CRISPR/Cas9 System
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Transfection, Isolation, and Screening of the CENP-E Knockout HeLa Cells
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Transfection, Isolation, and Screening of the CENP-E Knockout HeLa Cells
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Transfection, Isolation, and Screening of the CENP-E Knockout HeLa Cells

Centromere-Associated Protein-E Gene Editing Using the CRISPR/Cas9 System

DOI:
10.3791/201002-v

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04:10 min

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June 23, 2023

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, , , , , , , ,
1Department of Cell Biology and Genetics, The School of Basic Medical Sciences,Fujian Medical University, 2Key Laboratory of Stem Cell Engineering and Regenerative Medicine,Fujian Province University, 3College of Clinical Medicine for Obstetrics & Gynecology and Pediatrics,Fujian Medical University, 4Medical Research Center,Fujian Maternity and Child Health Hospital

Tags

Keywords: TransfectionHeLa CellsCENP-E KnockoutPlasmidTrypsin-EDTACell PassageReduced Serum MediumTransfection ReagentFluorescence MicroscopyCell CountingSerial DilutionPhenotype-based ScreeningDNA ExtractionPCRLigationTransformationSanger SequencingWild-typeMutant

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