JoVE
JoVE
Faculty Resource Center
Research
Behavior
Biochemistry
Biology
Bioengineering
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
JoVE Journal
JoVE Encyclopedia of Experiments
JoVE Chrome Extension
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to your Course
Authors
Librarians
High Schools
About
Sign-In
Sign In
Contact Us
Research
JoVE Journal
JoVE Encyclopedia of Experiments
Education
JoVE Core
JoVE Science Education
JoVE Lab Manual
High Schools
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Close
Research
Behavior
Biochemistry
Bioengineering
Biology
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
Products
JoVE Journal
JoVE Encyclopedia of Experiments
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
Products
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to Your Course
Teacher Resources
Get in Touch
Instant Trial
Log In
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Journal
/
Biology
/
Centromere-Associated Protein-E Gene Editing Using the CRISPR/Cas9 System
/
Immunofluorescence Staining and High-Resolution Confocal Microscopy of CRISPR/Cas9-Mediated
CENP-E
Knockout HeLa Cells
JoVE Journal
Biology
A subscription to JoVE is required to view this content.
Sign in or start your free trial.
JoVE Journal
Biology
Immunofluorescence Staining and High-Resolution Confocal Microscopy of CRISPR/Cas9-Mediated
CENP-E
Knockout HeLa Cells
Please note that all translations are automatically generated.
Click here for the English version.
Immunofluorescence Staining and High-Resolution Confocal Microscopy of CRISPR/Cas9-Mediated
CENP-E
Knockout HeLa Cells
Centromere-Associated Protein-E Gene Editing Using the CRISPR/Cas9 System
DOI:
10.3791/201003-v
•
03:07 min
•
June 23, 2023
•
Meng-Fei Xu
2
,
Jie Chen
2
,
Yue Xu
2
,
Jing-Lian Zhang
2
,
Yi Zhou
2
,
Jie-Jie He
2
,
Shan Wu
2
,
Ya-Lan Wei
4
,
Zhen-Yu She
2
1
Department of Cell Biology and Genetics, The School of Basic Medical Sciences
,
Fujian Medical University
,
2
Key Laboratory of Stem Cell Engineering and Regenerative Medicine
,
Fujian Province University
,
3
College of Clinical Medicine for Obstetrics & Gynecology and Pediatrics
,
Fujian Medical University
,
4
Medical Research Center
,
Fujian Maternity and Child Health Hospital
Tags
Keywords: Immunofluorescence Staining
Confocal Microscopy
CRISPR/Cas9
CENP-E
HeLa Cells
Cell Fixation
Cell Permeabilization
Primary Antibody
Secondary Antibody
DAPI
Chromosome Misalignment
Metaphase
Interphase
Prophase
Anaphase
Telophase
Embed
ADD TO PLAYLIST
Usage Statistics
Related Videos
check_url/201003?article_type=v
Генерация центромер-ассоциированных белков-E
CENP-E
-/-
нокаутных клеточных линий с использованием системы CRISPR/Cas9
check_url/201003?article_type=v
Transfection, Isolation, and Screening of the
CENP-E
Knockout HeLa Cells
check_url/201003?article_type=v
Immunofluorescence Staining and High-Resolution Confocal Microscopy of CRISPR/Cas9-Mediated
CENP-E
Knockout HeLa Cells
check_url/201003?article_type=v
Chromosome Preparation and Karyotype Analysis of CRISPR/Cas9-Mediated
CENP-E
Knockout HeLa Cells
Read Article