/

/

Centromere-Associated Protein-E Gene Editing Using the CRISPR/Cas9 System

/

Chromosome Preparation and Karyotype Analysis of CRISPR/Cas9-Mediated CENP-E Knockout HeLa Cells

JoVE Journal
Biology

A subscription to JoVE is required to view this content. Sign in or start your free trial.

JoVE Journal Biology

Chromosome Preparation and Karyotype Analysis of CRISPR/Cas9-Mediated CENP-E Knockout HeLa Cells

Please note that all translations are automatically generated. Click here for the English version.

Chromosome Preparation and Karyotype Analysis of CRISPR/Cas9-Mediated CENP-E Knockout HeLa Cells

Centromere-Associated Protein-E Gene Editing Using the CRISPR/Cas9 System

DOI:

10.3791/201004-v

•

02:19 min

•

June 23, 2023

•

Meng-Fei Xu², Jie Chen², Yue Xu², Jing-Lian Zhang², Yi Zhou², Jie-Jie He², Shan Wu², Ya-Lan Wei⁴, Zhen-Yu She²

¹Department of Cell Biology and Genetics, The School of Basic Medical Sciences,Fujian Medical University, ²Key Laboratory of Stem Cell Engineering and Regenerative Medicine,Fujian Province University, ³College of Clinical Medicine for Obstetrics & Gynecology and Pediatrics,Fujian Medical University, ⁴Medical Research Center,Fujian Maternity and Child Health Hospital

Tags

Keyword Extraction: Chromosome Preparation Karyotype Analysis CRISPR/Cas9 CENP-E Knockout HeLa Cells Colchicine Trypsin-EDTA Potassium Chloride Fixative Solution Giemsa’s Staining Light Microscope Plan Fluor Objective Chromosome Number Wild Type Mutant

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

Generatie van Centromeer-geassocieerde proteïne-E CENP-E^-/- knock-outcellijnen met behulp van het CRISPR/Cas9-systeem

Transfection, Isolation, and Screening of the CENP-E Knockout HeLa Cells

Immunofluorescence Staining and High-Resolution Confocal Microscopy of CRISPR/Cas9-Mediated CENP-E Knockout HeLa Cells

Chromosome Preparation and Karyotype Analysis of CRISPR/Cas9-Mediated CENP-E Knockout HeLa Cells

Read Article