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Plating Intact Patient-Derived Organoids e Configuração de Parâmetros de Imagem para Multiplexed Live-Cell Imaging
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Cancer Research
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JoVE Journal Cancer Research
Plating Intact Patient-Derived Organoids and Setting Up Imaging Parameters for Multiplexed Live-Cell Imaging

Plating Intact Patient-Derived Organoids e Configuração de Parâmetros de Imagem para Multiplexed Live-Cell Imaging

DOI:
10.3791/201045-v

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05:14 min

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January 05, 2024

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, , , , , , , , , , ,
1Department of Obstetrics and Gynecology, Carver College of Medicine,University of Iowa, 2Cancer Biology Graduate Program, Carver College of Medicine,University of Iowa, 3The Institute of Cancer Research: and the Royal Marsden NHS Foundation Trust, 4Department of Radiation Oncology, Carver College of Medicine,University of Iowa, 5Division of Molecular Medicine, Departments of Internal Medicine and Obstetrics and Gynecology,University of New Mexico Comprehensive Cancer Center, University of New Mexico Health Sciences Center, 6Agilent Technologies, 7Holden Comprehensive Cancer Center,University of Iowa

Tags

Patient-derived OrganoidsPDOBasement Membrane ExtractsBME96-well PlateLive-cell ImagingFluorescent DyesGen5 SoftwareImager Manual ModeZ-stackBrightfield ChannelFluorescent ChannelsGFPImage Pre-processingZ Projection

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