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Journal
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Cancer Research
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Plating Intact Patient-Derived Organoids e Configuração de Parâmetros de Imagem para Multiplexed Live-Cell Imaging
JoVE Journal
Cancer Research
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JoVE Journal
Cancer Research
Plating Intact Patient-Derived Organoids and Setting Up Imaging Parameters for Multiplexed Live-Cell Imaging
Plating Intact Patient-Derived Organoids e Configuração de Parâmetros de Imagem para Multiplexed Live-Cell Imaging
DOI:
10.3791/201045-v
•
05:14 min
•
January 05, 2024
•
Kaitriana E. Colling*
1,2
,
Emily L. Symons*
1
,
Lorenzo Buroni*
3
,
Hiruni K. Sumanasiri
,
Jessica Andrew-Udoh
,
Emily Witt
4
,
Haley A. Losh
,
Abigail M. Morrison
,
Kimberly K. Leslie
,
Christopher J. Dunnill
,
Johann S. de Bono
,
Kristina W. Thiel
7
1
Department of Obstetrics and Gynecology, Carver College of Medicine
,
University of Iowa
,
2
Cancer Biology Graduate Program, Carver College of Medicine
,
University of Iowa
,
3
The Institute of Cancer Research: and the Royal Marsden NHS Foundation Trust
,
4
Department of Radiation Oncology, Carver College of Medicine
,
University of Iowa
,
5
Division of Molecular Medicine, Departments of Internal Medicine and Obstetrics and Gynecology
,
University of New Mexico Comprehensive Cancer Center, University of New Mexico Health Sciences Center
,
6
Agilent Technologies
,
7
Holden Comprehensive Cancer Center
,
University of Iowa
Tags
Patient-derived Organoids
PDO
Basement Membrane Extracts
BME
96-well Plate
Live-cell Imaging
Fluorescent Dyes
Gen5 Software
Imager Manual Mode
Z-stack
Brightfield Channel
Fluorescent Channels
GFP
Image Pre-processing
Z Projection
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