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Isolation and Magnetic Separation of Murine CD34+ Stem Cells from the Vascular Wall
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Detección de señalización de calcio intracelular en células madre CD34+ de la pared vascular aisladas magnéticamente
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Medicine
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JoVE Journal Medicine
Detection of Intracellular Calcium Signaling in the Magnetically Isolated Vascular Wall CD34+ Stem Cells
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Detección de señalización de calcio intracelular en células madre CD34+ de la pared vascular aisladas magnéticamente

Isolation and Magnetic Separation of Murine CD34+ Stem Cells from the Vascular Wall

DOI:
10.3791/201056-v

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01:15 min

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December 22, 2023

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, , , , , , ,
1Key Laboratory of Medical Electrophysiology of Ministry of Education and Medical Electrophysiological Key Laboratory of Sichuan Province, Institute of Cardiovascular Research,Southwest Medical University

Tags

Immunomagnetic IsolationVascular Wall-resident CD34+ Stem CellsPurificationFlow CytometryFunctional IdentificationImmunofluorescence StainingCa2+ ImagingMurine Aorta And Mesenteric ArteryAdventitiaCell CultureMultipotencyDifferentiationATPCaffeineThapsigargin

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