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Journal
/
Immunology and Infection
/
Quantifying NET Formation Using Dual-Dye Live Cell Analysis
/
Human Neutrophil Staining and Preparation of Neutrophil Assay Plate for Live Cell Analysis
JoVE Journal
Immunology and Infection
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JoVE Journal
Immunology and Infection
Human Neutrophil Staining and Preparation of Neutrophil Assay Plate for Live Cell Analysis
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Human Neutrophil Staining and Preparation of Neutrophil Assay Plate for Live Cell Analysis
Quantifying NET Formation Using Dual-Dye Live Cell Analysis
DOI:
10.3791/201217-v
•
01:37 min
•
December 01, 2023
•
Shuichiro Nakabo
,
Mariana J. Kaplan
,
Sarthak Gupta
1
Systemic Autoimmunity Branch, National Institute of Arthritis and Musculoskeletal and Skin Diseases (NIAMS)
,
National Institutes of Health (NIH)
Tags
Neutrophil Staining
Neutrophil Isolation
Neutrophil Assay
Live Cell Analysis
DNA Dye
Membrane Permeable Dye
Membrane Impermeable Dye
Hemocytometer
Cell Counting
Cell Suspension
RPMI Medium
Cell Stimulation
Cell Culture Plate
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