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Journal
/
Developmental Biology
/
Enhanced 3D Neural Retina Differentiation from Human Embryonic Stem Cells
/
Cultivo de células-tronco embrionárias H9 para geração de retina neural humana
JoVE Journal
Developmental Biology
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JoVE Journal
Developmental Biology
Culturing H9-Embryonic Stem Cells for Generation of Human Neural Retina
Cultivo de células-tronco embrionárias H9 para geração de retina neural humana
Enhanced 3D Neural Retina Differentiation from Human Embryonic Stem Cells
DOI:
10.3791/201241-v
•
02:03 min
•
December 22, 2023
•
Wen Li*
1,2
,
Hongyu Li*
3
,
Hao Yan
,
Lixiong Gao
,
Xiaoling Wang
,
Lingping Zhao
,
Yuhan Yan
2
,
Zi Ye
,
Jiafei Xi
,
Wen Yue
,
Zhaohui Li
2
1
Medical School of Chinese PLA
,
2
Senior Department of Ophthalmology
,
The Third Medical Center of Chinese PLA General Hospital
,
3
Stem Cell and Regenerative Medicine Lab
,
Beijing Institute of Radiation Medicine
Tags
Keywords: H9-Embryonic Stem Cells
Neural Retina
Extracellular Matrix
Maintenance Medium
Y-27632
Centrifugation
Cell Culture
Cell Density
EDTA
PBS
Incubation
Carbon Dioxide
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